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gmuC [2018-06-22 17:47:01]
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gmuC [2018-06-22 17:47:01]

glucomannan-specific phosphotransferase system, EIIC component of the PTS
locus
BSU05830
pI
9.80
mw
48.27 kDa
protein length
442 aa Sequence Blast
gene length
1326 bp Sequence Blast
function
glucomannan uptake and phosphorylation
product
glucomannan-specific phosphotransferase system, EIIC component
essential
no
synonyms
ydhO
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]

    • Gene

    Coordinates
    627,284 → 628,612

    The protein

    Protein family

  • [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, lactose permease (Lac) family [Pubmed|10627040]

    • Paralogous protein(s)

  • [protein|83A7C160B0301A2B51B65478D99ACB753D67AF74|LicC], [protein|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|YwbA]

    • Structure

  • [PDB|3QNQ] (EIIC component of diacetylchitobiose specific PTS from ''Bacillus cereus''; 41% identity, 80% similarity) [Pubmed|21471968]

    • Expression and Regulation

    Operons

    genes
    [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
    description
    [Pubmed|18177310]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]
    • view in new tab

    additional information

  • [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

    • Biological materials

    Mutant

  • MGNA-C190 (ydhO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2188 NBRP B. subtilis, Japan]
  • BKE05830 (Δ[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAACACCCTATCCACCCCGA, downstream forward: _UP4_ATGTGACATAAGGGGAGAGA
  • BKK05830 (Δ[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAACACCCTATCCACCCCGA, downstream forward: _UP4_ATGTGACATAAGGGGAGAGA

    • References

  • 18177310,10627040,20817675,21471968